Determining MATH and BTB containing genes in the genome of the pumpkin (Cucurbita pepo L.)

Gulin, Maja (2009) Determining MATH and BTB containing genes in the genome of the pumpkin (Cucurbita pepo L.). Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

MATH-BTB domain-containing proteins (MAB) are essentially important in a variety of species during the time of embryogenesis and post-embryonic development. Also, MAB proteins are involved in establishing the order within the cortical microtubule network which builds the spindle during the cell division, and increase the specificity of cullin E3 ligase against different substrates. Because of its importance, the aim of this study was to investigate the existence of genes with the MATH and BTB domains in the C. pepo genome. Using the gene sequence of the BPM1 gene from A. thaliana, the probes for the Southern hybridization were constructed and MAB genes were identified in C. pepo. Based on similarities between different MAB gene sequences, primers were constructed for amplification of homologuos genes in pumpkin. Using genomic DNA as template, intergenic spacers of ribosomal genes were amplified, and using complementary DNA from embriogenic pumpkin tissue as template, no gene was amplified.

Item Type: Thesis (Diploma thesis)
Keywords: protein domains MATH and BTB, MEL-26, BPM, C. pepo, cullin3-based E3 ligase, TaMAB proteins
Supervisor: Bauer, Nataša
Date: 2009
Number of Pages: 61
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 09 Sep 2014 10:58
Last Modified: 09 Sep 2014 10:58
URI: http://digre.pmf.unizg.hr/id/eprint/2673

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