The efficiency of DNA repair in Escherichia coli ssb-1 mutant

Perković, Sanja (2009) The efficiency of DNA repair in Escherichia coli ssb-1 mutant. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Damaged DNA in Escherichia coli can be repaired by two main pathways. RecBCD pathway repairs double stranded breaks. The essential enzyme this pathway uses is RecBCD which has helicase, exonuclease activity, and the capability of loading RecA protein onto single stranded DNA. RecF pathway repairs single stranded gaps made in DNA molecule. It does not have a single enzyme performing all the essential activities. RecQ, RecJ, RecF, RecO and RecR proteins are required for polimerization of RecA nucleoprotein filament on single stranded DNA. The lost activities (nuclease and RecA loading) are compensated by combining both pathways of recombination in recB1080 mutant. Mutation ssb-1 produces temperature unstable SSB-1 protein which has a lower affinity of binding to single stranded DNA. In this thesis we wanted to see if lower binding affinity of SSB-1 protein to ssDNA will result in more efficient replacement with RecA protein. Results indicate that ssb-1 mutation inhibits the activity of RecBCD enzyme. RecFOR proteins are very important for the exchange of SSB-1 tetramere with RecA protein.

Item Type: Thesis (Diploma thesis)
Keywords: Escherichia coli, homologous recombination, RecBCD, RecF, ssb-1, DNA repair
Supervisor: Ivančić Baće, Ivana
Date: 2009
Number of Pages: 36
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 09 Sep 2014 12:01
Last Modified: 17 Sep 2014 12:57
URI: http://digre.pmf.unizg.hr/id/eprint/2678

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