Binding of CTCF and BORIS to TOP2A promoter in Cal 27 cells

Ćuk, Katarina (2009) Binding of CTCF and BORIS to TOP2A promoter in Cal 27 cells. Diploma thesis, Faculty of Science > Department of Biology.

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The enzyme topoisomerase IIα (encoded by TOP2A) is essential for cell survival. Its expression is often upregulated in malignant tumors and has been implicated in cellular sensitivity to cytotoxic drugs. Protein CTCF and its paralogue BORIS, which is normally expressed only in a subpopulation of spermatocytes and aberrantly in various tumors, have important roles in the establishment and maintenance of DNA methylation and transcriptional regulation. Since those two proteins bind to the same DNA motifs, but have different roles in transcriptional regulation, the aim of this study was to investigate their association with the TOP2A promoter. With an 3-aminobenzamide treatment I have induced BORIS in Cal 27 cells and by chromatin immunoprecipitation demonstrated that CTCF and BORIS bind to the TOP2A promoter. Results of this study facilitate further investigation of their role in the regulation of TOP2A transcription and the applicability of its modulation in the therapy of malignant tumors.

Item Type: Thesis (Diploma thesis)
Keywords: TOP2A promoter, CTCF, BORIS, chromatin immunoprecipitation
Supervisor: Novak-Kujundžić, Renata
Co-supervisor: Oršolić, Nada
Date: 2009
Number of Pages: 50
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 09 Sep 2014 12:20
Last Modified: 09 Sep 2014 12:20

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