Characterization of ubiquitin-binding domain in C1orf124 protein

Burić, Duje (2009) Characterization of ubiquitin-binding domain in C1orf124 protein. Diploma thesis, Faculty of Science > Department of Biology.

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Ubiquitin has recently been discovered to have a key role in a variety of biological processes. In parallel, different protein domains were identified with the ability to bind different ubiquitin species. Ubiquitin-binding zinc-finger 4 (UBZ4) family was recently revealed using bioinformatical analysis. UBZ4 is a C2HC Zn-finger found in all eukaryotic lineages; most of the UBZ4-containing proteins appear to play a role in the DNA damage response. Iterative alignment with known UBZ4 revealed 13 members of putative family. C1orf124 protein was chosen to analyse its ubiquitin binding abilities. I have showed that C1orf124 protein binds monoubiquitin and polyubiquitin chains. Pull down assay showed that interaction is achieved through hydrophobic patch surrounding Ile44 on ubiquitin and Asp residue in-between second Zn-binding dyad of UBZ4 domain.

Item Type: Thesis (Diploma thesis)
Keywords: Ubiquitin, UBD, UBZ4, DNA repair, metalloprotease SprT family
Supervisor: Đikić, Ivan
Co-supervisor: Matulić, Maja
Date: 2009
Number of Pages: 61
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 10 Sep 2014 09:23
Last Modified: 10 Sep 2014 09:23

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