Structural alterations of selected genes in human testicular germ cell tumours

Vladušić, Tomislav (2013) Structural alterations of selected genes in human testicular germ cell tumours. Doctoral thesis, Faculty of Science > Department of Biology.

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Testicular germ cell tumors are the most common malignacies in young males. Two histological groups, seminoma and nonseminoma, differ morphologically and in malignant behaviour. Although a common cytogenetic feature is seen, amplification of 12p chromosomal region, development mechanisms of weakly aggressive seminoma and more aggressive nonseminoma are unknown. Molecular biology studies of these tumors are often contradictory. Occurence of structural genetic alterations was analysed in 18 seminoma and 22 nonseminoma for genes involved in a malignant tumour phenotype: CDH1, APC (cellular differentiation and adhesion); NME1 (cellular motility); TP53, CDKN2A, RB1 (cell cycle and genome integrity control); RAD51, MSH2, MLH1, BRCA1 (DNA damage repair); BAX (apoptosis); ABCG2 (resistance to xenobiotics). Genetic alterations, loss of heterozygosity (LOH) and microsatellite instability (MSI), were analysed using restriction fragments length or microsatellite repeats length polymorphisms. A difference betwen structural genetic alterations in seminoma and nonseminoma has been observed. Occurence of genetic alterations indicate an independent development of seminoma and nonseminoma, and clearly correlates with clinical behaviour of those tumours.

Item Type: Thesis (Doctoral thesis)
Keywords: testicular germ cell tumors, seminoma, nonseminoma, loss of heterozygosity
Supervisor: Hrašćan, Reno
Date: 2013
Number of Pages: 115
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 27 Jan 2014 13:43
Last Modified: 22 Sep 2014 19:51

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