Expression level of TLR9 gene in human tumour tissues

Pongrac, Igor (2009) Expression level of TLR9 gene in human tumour tissues. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns and enable innate immune responses. They are also expressed on tumour cells from a wide variety of tissues, suggesting that TLR activation may be an important event in tumour cell immune evasion. The aim of this study was to determine whether TLR9 expression is associated with disease manifestation in lung and hypophyseal tumours. Gene TLR9 expression was evaluated by real-time quantitative RT-PCR. Total mRNA from 45 hypophyseal tumour tissue specimen, 20 lung tumour and 20 normal lung tissue specimen was isolated, purified and retrotranscribed. cDNA was analysed measuring TLR9 relative expression using TaqMan probe technology. Results of the analysis of hypophyseal tumor tissue samples showed the presence of different levels of TLR9 gene expression: ranging from very high in 2 samples (4,4%) to distinctively low in 9 samples (20%). Results of the analysis of 20 lung tumor tissue samples and associated healthy tissues showed higher expression of TLR9 gene in 8 (40%) tumor tissues, in 8 samples (40%) the expression was decreased and in 4 samples (20%) it was unchanged, compared to healthy tissues.

Item Type: Thesis (Diploma thesis)
Keywords: toll-like receptor 9, gene expression, tumour, hypophysis, lung
Supervisor: Pavelić, Jasminka
Co-supervisor: Oršolić, Nada
Date: 2009
Number of Pages: 39
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 10 Sep 2014 09:56
Last Modified: 10 Sep 2014 09:56
URI: http://digre.pmf.unizg.hr/id/eprint/2691

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