Identification of genotoxic potential of surface water and sediment samples of the Sava river by Ames biotest

Crnjak, Martina (2009) Identification of genotoxic potential of surface water and sediment samples of the Sava river by Ames biotest. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

The main goal of the NATO SfP project entitled Assessment of Hazardous chemical contaminatin in the Sava River basin was to develop and implement a modern, EDA (Effects Directed Analysis) approach customed for monitoring and risk assessment of hazardous chemical contamination in the Sava River basin. EDA approach uses the combination of biological assays (biotests) and analytical chemical detection and separation technique to direct the identification of contaminants causing toxic effects. Within a series of biotests used in the project, the Ames bacterial test was applied as the most widely used assay for assessment of mutagenicity of tested compounds or complex environmental samples. Test was performed using genetically modified strains of Salmonella typhimurium, and for detection of premutagens (substances that express mutagenicity upon enzymatic bioactivation) the postmitochondrial fraction of rat liver was used as activation system. Our results clearly showed that the analyzed samples of sediments do not possess the mutagenic potential, while some surface and waste water samples showed threshold values in respect to internationally accepted criteria for mutagenicity.

Item Type: Thesis (Diploma thesis)
Keywords: Ames test, genotoxicity, biotransformation, EDA
Supervisor: Smital, Tvrtko
Date: 2009
Number of Pages: 53
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 10 Sep 2014 12:15
Last Modified: 10 Sep 2014 12:15
URI: http://digre.pmf.unizg.hr/id/eprint/2701

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