Determination of thallium(I) acetate genotoxicity in common duckweed Lemna minor L. by comet assay

Brnjilović, Petar (2009) Determination of thallium(I) acetate genotoxicity in common duckweed Lemna minor L. by comet assay. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Concentration of the heavy metal thallium in the environment has been continuously increasing. Since thallium can be absorbed by plants it becomes available for the higher organisms including men through food chains. The main goal of this work was to determine genotoxicity of thallium(I) acetate by comet assay as a non-specific genotoxicity biomarker in common duckweed Lemna minor L. Duckweed cell nuclei in in vitro conditions were treated with hydrogen peroxide as a model genotoxicant in order to verify the comet assay sensitivity. Results are showing direct genotoxic effect. Common duckweed plants were in vivo exposed to different thallium(I) acetate concentrations for seven days. The results of comet assay showed a mild genotoxicity of two highest concentrations of thallium. In order to determine whether thallium directly or indirectly affect DNA an acellular comet assay was performed in vitro on common duckweed nuclei. Results following both cellular and acellular comet assays implies indirect DNA damage caused by thallium(I) acetate.

Item Type: Thesis (Diploma thesis)
Keywords: Lemna minor L., thallium(I) acetate, comet assay, hydrogen peroxide, genotoxicity
Supervisor: Pavlica, Mirjana
Date: 2009
Number of Pages: 45
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 11 Sep 2014 09:36
Last Modified: 11 Sep 2014 09:36
URI: http://digre.pmf.unizg.hr/id/eprint/2708

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