Determination of sediment toxicity of Gorjak stream by bioassays in vitro

Šestak, Ivana (2009) Determination of sediment toxicity of Gorjak stream by bioassays in vitro. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Numerous persistent organic pollutants (POPs), such as polychlorinated dibenzo-p-dioxins and furans (PCDD/F), polychlorinated biphenyls (PCBs), polychlorinated naphthalenes (PCNs) and polyaromatic hydrocarbons (PAHs) are highly toxic. POPs get into environment mostly by industrial wastewaters. By releasing untreated industrial wastewater into the Gorjak stream, Industries Pliva Hrvatska d.d. and Kvasac d.o.o. for decades represent a problem for local community. The purpose of this research was to determine ecotoxicity of Gorjak stream sediment samples, using two bioassays: (1) chronic toxicity test (growth inhibition test with green algae Scenedesmus subspicatus), and (2) EROD test for determination of the activity of the mixed function oxygenases cellular detoxification system. Using chronic toxicity test we measured sample dilutions at which 50% growth inhibion of the algae was achieved (IC50) in comparison to the control group. EC50 values representing corresponding amount of sediment were determined and toxicity units (TU) obtained recalculated by expression TU = 100 / EC50. For the EROD bioassay we calculated TCDD toxicity equivalents (TCDD TEQ). Results showed high sediment toxicity at each location, with the highest values determined at location closest to the wastewater outlet of the above mentioned industrial facilities.

Item Type: Thesis (Diploma thesis)
Keywords: POPs, bioassays, chronic toxicity, EROD, TCDD TEQ
Supervisor: Smital, Tvrtko
Co-supervisor: Klobučar, Goran
Date: 2009
Number of Pages: 60
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 11 Sep 2014 10:17
Last Modified: 17 Sep 2014 13:00
URI: http://digre.pmf.unizg.hr/id/eprint/2709

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