Selectivity of commercial solid media for isolation of total coliform bacteria from surface water

Šimunović, Senka (2009) Selectivity of commercial solid media for isolation of total coliform bacteria from surface water. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Selectivity of commertial media for isolation of total coliform bacteria from surface water, was investigated on sample of clean culture of Escherichia coli and 19 samples of water, collected at seven brooks of Zagreb, at lake of Botanical Garden and five lakes of Maksimir. Samples was inoculated on investigated media with Plate Strake Method. The aim of this research was to investigate efficiency of isolation of total coliform bacteria and Escherichia coli on four commertial solid media: EC-X Gluc agar, Endo agar, m-Faecal Coliform agar and MacConkey agar. The data comparison established that the media are different by the number of grown colonies. MacConkey agar is media where the most colonies were grown, this is at the same time the best media for isolation of total coliform bacteria. The following two are Endo agar and m-Faecal Coliform agar, which results are not that different. The worst medium for isolation of coliform bacteria is EC-X Gluc agar.Because the media are differential, the colonies of Escherichia coli were separately counted. The results show that the most selective medium for isolation of Escerichia coli is MacConkey agar. It's followed by m-Faecal Coliform agar and Endo agar. The worst medium for isolation of Escerichia coli is EC-X Gluc agar.

Item Type: Thesis (Diploma thesis)
Keywords: coliform bacteria, Escherichia coli, solid media
Supervisor: Hrenović, Jasna
Date: 2009
Number of Pages: 48
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 11 Sep 2014 11:03
Last Modified: 11 Sep 2014 11:03
URI: http://digre.pmf.unizg.hr/id/eprint/2714

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