Retargeting adenovirus type 5 to integrin α4β1

Jelušić, Tihana (2009) Retargeting adenovirus type 5 to integrin α4β1. Diploma thesis, Faculty of Science > Department of Biology.

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Genetic retargeting of Adenovirus type 5 (Ad5) through insertion of sequences in the fiber protein should permit targeted gene delivery. The aim of this research is to construct replication defective adenoviral vector by incorporating α4β1 integrin-targeting ligand CPLDIDFYC into the HI-loop of the fiber protein, in order to retarget Ad5 to integrin α4β1 (also known as very late antigen 4, VLA-4). Construction of Ad5VLA-4 was performed by manipulation of the full length Ad5 genome as a stable plasmid in E. coli, using the bacterial homologous recombination machinery. Ad5VLA-4 and a control virus Ad5wt lacking targeting sequence, were multiplied in human embryonic kidney cell line-293 and purified by bending in CsCl gradients. On the rhabdomyosarcoma (RD) cell line, which express α4β1 integrin, Ad5VLA-4 exhibited increased transduction efficacy in comparison to Ad5wt. Therefore, Ad5VLA-4 could be suitable vector for gene therapy of tumors expressing α4β1 integrin.

Item Type: Thesis (Diploma thesis)
Keywords: Ad retargeting, integrin α4β1, VLA-4
Supervisor: Ambriović Ristov, Andreja
Date: 2009
Number of Pages: 44
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 11 Sep 2014 11:16
Last Modified: 11 Sep 2014 11:16

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