HOXB13 and its downstream targets in ovarian cancer progression

Šuštić, Tonći (2009) HOXB13 and its downstream targets in ovarian cancer progression. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

The HOX genes have been implicated in different aspects of the oncogenic process, as ectopic expression of some HOX genes promotes cellular transformation in vitro and tumorigenesis in vivo. The observation that HOXB13 is expressed in a high percentage of breast and ovarian cancer cell lines and tumors, and that the expression of HOXB13 is associated with a more aggressive clinical course, led us to investigate the ectopic expression of HOXB13 in genetically defined ovarian cancer cell lines. We report identification of several putative HOXB13 targets among which clusterin takes the most prominent role. Clusterin is present in two distinct isoforms, as secreted clusterin (sCLU) and as nuclear clusterin (nCLU). While the former is associated with cell survival, the latter is pro-apoptotic. As a part of the project aiming to identify new therapeutic targets for ovarian cancer, here we describe the generation of clusterinoverexpressing cell lines, representing two contradictory functions of clusterin. These cell lines have subsequently been analyzed using both in vitro and in vivo assays. Consequently, stable ectopic expression of both clusterin isoforms has been demonstrated.

Item Type: Thesis (Diploma thesis)
Keywords: ovarian cancer, mouse model of ovarian cancer, HOXB13, clusterin
Supervisor: Oršulić, Sandra
Co-supervisor: Oršolić, Nada
Date: 2009
Number of Pages: 51
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 11 Sep 2014 11:26
Last Modified: 17 Sep 2014 13:03
URI: http://digre.pmf.unizg.hr/id/eprint/2718

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