Rooting and acclimatization of black chokeberry (Aronia melanocarpa Michx.) shoots

Črnjević, Silvija (2009) Rooting and acclimatization of black chokeberry (Aronia melanocarpa Michx.) shoots. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Black chokeberry (Aronia melanocarpa Michx.) is a deciduous bush-like plant which belongs to the Rosaceae family. The species was introduced in in vitro culture due to high atioxidative activity of their fruits. Rooting and subsequent acclimatization of black chokeberry has been investigated on MS nutrient media containing different concentration of macronutrients (MS, ½ MS, ¼ MS). The highest rooting percentage (85.4%) and the best morphological characteristics were obtained on ½ MS medium. Therefore, the research was continued on ½ MS, supplemented with auxins α- naphthaleneacetic acid (NAA) or indole-3-butyric acid (IBA). Auxins NAA or IBA inhibited the main root induction, but stimulated adventitious root formation from the callus. Maximum adventitious rooting occured in shoots grown on ½ MS containing 1.5 mg/L NAA (95.8%) or 1.0 mg/L IBA (100%). Acclimatization of in vitro rooted microcuttings was equally successful (80-90%) in plants potted to commercial peat substrate (COMPO SANA) and mixture of pumice, tenisit and peat (3:3:1). Aronia melanocarpa microcuttings without roots exhibited poor ex vitro rooting and low acclimatization percentage (50-60%).

Item Type: Thesis (Diploma thesis)
Keywords: Aronia melanocarpa, micropropagation, MS, rooting, acclimatization
Supervisor: Pevalek-Kozlina, Branka
Co-supervisor: Babić, Marija
Date: 2009
Number of Pages: 40
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 11 Sep 2014 12:09
Last Modified: 11 Sep 2014 12:09
URI: http://digre.pmf.unizg.hr/id/eprint/2723

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