Phylogeny of Austropotamobius pallipes (Lereboullet, 1858) based on the analysis of 16S and COI mitochondrial DNA markers

Futo, Momir (2009) Phylogeny of Austropotamobius pallipes (Lereboullet, 1858) based on the analysis of 16S and COI mitochondrial DNA markers. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Phylogenetic relations within populations of Austropotamobius pallipes (Lereboullet, 1858) are yet not fully understood. Recent research reveals a possible existence of speciescomplex, that is, a multiple taxa existence within a single species that is today known as Austropotamobius pallipes. The aim of this study is to verify that hypothesis and if necessary suggest a differentiation of the species-complex in to two separate species. The second intention is to put the Croatian crayfish in to the right phylogenetic context. Phylogenetic relations between 76 A. pallipes individuals, sampled from 22 localities in Croatia, Bosnia and Herzegovina and Austria were studied in this graduation thesis. The analysis of A. pallipes populations is based on DNA sequence variations of mitochondrial genes for 16S rRNA and COI. The extracted gene sequences were compared with homologues sequences that were used in other studies of this species all over Europe. Phylogenetic studies were carried out using statistical phylogenetic analysis by means of different character state and distance matrix methods. The results of this research showed that the species-complex Austropotamobius pallipes consists of two distinct species and a few subspecies. Also they are a contribution to a better understanding of relations between Croatian and European populations and they specify the time of divergence within population in the Southeast Balkan Peninsula.

Item Type: Thesis (Diploma thesis)
Keywords: phylogeny, Austropotamobius pallipes, 16S rRNA, COI, mitochondrial DNA
Supervisor: Maguire, Ivana
Co-supervisor: Franjević, Damjan
Date: 2009
Number of Pages: 90
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 12 Sep 2014 09:52
Last Modified: 12 Sep 2014 09:52
URI: http://digre.pmf.unizg.hr/id/eprint/2734

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