The effects of heavy metals on esterase activity of duckweed (Lemna minor L.)

Čavka, Tanja (2009) The effects of heavy metals on esterase activity of duckweed (Lemna minor L.). Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Duckweed (Lemna minor L.) is a widely spread monocot often used as a model organism in ecotoxicological studies. The effects of heavy metals present in certain surface and wastewater samples on esterase activity and isoesterase patterns of duckweed cultured in vitro were investigated. Duckweed plants were exposed to surface - Sava Županja, Sava Jasenice and Sutla Prišlin (collected monthly over a 3-month monitoring period) and wastewater samples - ZGOS (collected every 4h during one day) and Jakuševac for seven days. The contents of zinc, mercury and chromium were elevated while those of iron, nickel, cadmium, arsenic and manganese were below the maximum allowed value. Esterase activities, measured with 2-naphtylacetate, increased in duckweed exposed to ZGOS (1-3), Jakuševac, SŽ (1-3) and SJ (2-3) water samples. In total, three isoforms of esterase were present in both control and treated duckweed and appearance of new bands was not noticed. The content of soluble proteins was decreased in duckweed exposed to all tested water samples though especially in plants exposed to ZGOS. Results obtained indicate a possible involvement of esterases in duckweed's adaptation to stress caused by heavy metals. Esterase activity could be used as a potential marker of toxicity induced by various agents.

Item Type: Thesis (Diploma thesis)
Keywords: esterase activity, Lemna minor L., heavy metals, soluble proteins
Supervisor: Pevalek-Kozlina, Branka
Date: 2009
Number of Pages: 39
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 12 Sep 2014 10:44
Last Modified: 17 Sep 2014 12:59
URI: http://digre.pmf.unizg.hr/id/eprint/2740

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