Intramolecular recombination in bacteria Escherichia coli and Agrobacterium tumefaciens

Bočkor, Luka (2008) Intramolecular recombination in bacteria Escherichia coli and Agrobacterium tumefaciens. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Tandemly repeated DNA sequences are common in the genomes of many organisma and are susceptive to deletions and amplifications. That way they are a major cause of genome instability. Genetic analysis of repeated sequence rearrangements in Escherichia coli has revealed that multiple mechanisms participate in the process. They can be roughly divided into RecA-dependant and RecA-independent mechanisms. In this graduation thesis we wanted to test intramolecular recombination efficiency of bacterium Agrobacterium tumefaciens and compare it to that in Escherichia coli. For that purpose we have designed a plasmid which has tandem repeats of incomplete spectinomycin genes separated with ampicillin resistance gene. Spectinomycin resistance can be achieved either by RecA-dependant homologous recombination between direct repeats, while simultaneously deleting ampicillin resistance gene, or by RecAindependent mechanisms, which yields both ampicillin resistance gene and spectinomycin resistance genes amplification as a product. In this graduation thesis we have shown that intramolecular recombination in A. tumefaciens is mostly RecAindependent.

Item Type: Thesis (Diploma thesis)
Keywords: tandem repeats, recombination, Agrobacterium tumefaciens, Escherichia coli, RecA
Supervisor: Ivančić Baće, Ivana
Co-supervisor: Malenica, Nenad
Date: 2008
Number of Pages: 53
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 12 Sep 2014 11:48
Last Modified: 12 Sep 2014 11:48
URI: http://digre.pmf.unizg.hr/id/eprint/2744

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