Differential and Cytochemical Analysis of Blood Cells in Green Frogs (Pelophylax ridibundus, Pallas, 1771)

Kleiner, Iva (2013) Differential and Cytochemical Analysis of Blood Cells in Green Frogs (Pelophylax ridibundus, Pallas, 1771). Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

This thesis presents results attained from peripheral blood of the frog Pelophylax ridibundus. It contains hematocrit values, erythrocytes and leukocytes counts. The May-Grünwald Giemsom stain was used in morphological and quantitative blood cell analysis. Cytological characterization of leukocytes types used differential cytochemical stains. The alcian blue (AB) dye and the periodic acid Schiff method (PAS) stained glycoconjugates and polysaccharides. The sudan black B (SBB) dye stained cell lipids. Activities of alpha-naphthyl acetate esterase (ANAE), alpha-naphthyl butyrate esterase (ANBE) and alkaline phosphatase (ALP) were proved using enzyme-cytochemical reactions. Leukocyte count and percentage, hematocrit and cell morphology are similar to other frogs, unlike erythrocyte counts which are greater. AB stained nuclei of all cells, cytoplasm of lymphocytes, monocytes, some neutrophils, thrombocytes and some eosinophil granules. PAS stained lymphocytes, eosinophil granules, some monocytes, neutrophils and thrombocytes. All leukocytes, thrombocytes, young and mature erythrocytes stained with SBB. Monocytes and lymphocytes have active ANAE. Monocytes, some lymphocytes and thrombocytes have active ANBE. ALP activity was not recorded.

Item Type: Thesis (Diploma thesis)
Keywords: amphibian, dye, erythrocyte, leukocyte, hematology, stain, thrombocyte
Supervisor: Đikić, Domagoj
Date: 2013
Number of Pages: 63
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 28 Jan 2014 14:07
Last Modified: 22 Sep 2014 15:49
URI: http://digre.pmf.unizg.hr/id/eprint/277

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