HLA microsatellites polymorphism among recipient-donor pairs in hematopoietic stem cell transplantation

Rimac, Marina (2013) HLA microsatellites polymorphism among recipient-donor pairs in hematopoietic stem cell transplantation. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

In this thesis we analyzed the influence of six HLA microsatellites (D6S1014, D6S273, TNFa, D6S2927, D6S2793, D6S510) on the patients' survival after hematopoietic stem cells transplantation (HSCT). DNA of 50 recipient-unrelated donor pairs, included in the study, was isolated from peripheral blood. Microsatellites analysis was performed using polymerase chain reaction and electrophoresis on a polyacrylamide gel in an automated sequencer. D6S2927 microsatellite demonstrated a two-allele match for the majority of tested pairs (91.5%). Conversely, TNFa locus showed one-allele match and no match in most cases (44.7% and 10.6%, respectively). Analysis of allele matching association with patient's survival showed a statistical significance only for D6S2793 (P<0.05). Patients with two D6S2793 allele matches showed a better survival compared to patients with one or no allele matched. Based on this we can suggest that the region in the proximity of D6S2793 microsatellite is of importance to the patient's survival after unrelated HSCT.

Item Type: Thesis (Diploma thesis)
Keywords: hematopoietic stem cell transplantation, unrelated donor, HLA microsatellites, patients' survival
Supervisor: Grubić, Zorana
Date: 2013
Number of Pages: 31
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 26 Sep 2014 08:20
Last Modified: 26 Sep 2014 08:20
URI: http://digre.pmf.unizg.hr/id/eprint/2830

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