Genotoxic effects of gold and silver nanoparticles on earthworm Eisenia fetida (Savigny, 1826)

Lisjak, Damir (2013) Genotoxic effects of gold and silver nanoparticles on earthworm Eisenia fetida (Savigny, 1826). Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

In the recent years use of nanotechnology has increased, including many new materials containing nanoparticles. Nanoparticles are defined as materials smaller than 100 nm, in one dimension at least. Their biological activity is not yet fully known. Due to their small size, but large surface area, nanoparticles can induce oxidative stress and genotoxic effect. In this study we have measured the genotoxic influence of gold and silver nanoparticles on earthworms' coelomocytes (Eisenia fetida, Savigny 1826) using alkaline comet assay. For the assessment of genotoxic effects of nanoparticles of particular metal, it is important to gain insight into the genotoxicity of its ionic forms. For this purpose genotoxicity of silver and gold salts (AgNO3, KAuCl4) was also measured. Exposure lasted 10 days and it was conducted under controlled laboratory conditions in May 2012. DNA damage showed that ionic forms of gold and silver nanoparticles are more harmful than nanoparticles in elemental state.

Item Type: Thesis (Diploma thesis)
Keywords: Eisenia fetida, coelomocytes, comet-assay, nanoparticles, gold, silver
Supervisor: Klobučar, Goran
Date: 2013
Number of Pages: 31
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 26 Sep 2014 09:18
Last Modified: 26 Sep 2014 09:18
URI: http://digre.pmf.unizg.hr/id/eprint/2831

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