Detection of variant Ph chromosome translocations by interphasic fluorescence hybridization in situ (FISH)

Jurić, Magdalena (2013) Detection of variant Ph chromosome translocations by interphasic fluorescence hybridization in situ (FISH). Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Chronic myeloid leukemia (CML) is a myeloproliferative disorder characterized by the presence of the Philadelphia (Ph) chromosome, which is the result of the reciprocal translocation between chromosome 9 i 22, t(9;22)(q34;q11). The molecular consequence of this translocation is constitutively active protein tyrosine kinase BCR-ABL1, which causes CML by phosphorylating numerous downstream proteins involved in cell growth and apoptosis. It is considered that the vast majority of patients with CML show classical t(9;22)(q34q11) translocation and that in 5-10% CML cases variant Ph translokations are present. In these cases, one or more additional chromosomes are added to 9 and 22 chromosomes in translocation. The aim of this paper is to detect variant Ph chromosome translocations by interphasic fluorescence hybridization in situ (I-FISH) in patients with chonic myeloid leukemia at diagnosis, using LSI BCR/ABL Dual Color, Dual Fusion probe.

Item Type: Thesis (Diploma thesis)
Keywords: CML, BCR-ABL1 gene, variant Ph chromosome translocations, FISH, probe
Supervisor: Davidović-Mrsić, Sanja
Co-supervisor: Inga, Marijanović
Date: 2013
Number of Pages: 49
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 26 Sep 2014 10:15
Last Modified: 26 Sep 2014 10:15
URI: http://digre.pmf.unizg.hr/id/eprint/2833

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