Outcome of abiotic stress on germination and growth of transgenic seedlings Arabidopsis thaliana with increased expression of genes BPM1 and SerRS

Kolano, Sanja (2013) Outcome of abiotic stress on germination and growth of transgenic seedlings Arabidopsis thaliana with increased expression of genes BPM1 and SerRS. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

In previous researches the transgenic lines of the model plant Arabidopsis thaliana had been prepared and transformed with genes BPM1 (AT5G19000.1) and SerRS (AT5G27470) which are part of homeostasis of proteins. The BPM1 protein is adapter for ubiquitin ligase cullin 3 and it participates in the process of marking the proteins for degradation by proteasome 26S. The SerRS protein is seryl-tRNA synthetase which participates in process of protein synthesis. Recent research of BPM1 and SerRS genes expression indicates that these two genes have function during stress. In this thesis the function of BPM1 and SerRS proteins are characterized during salt and osmotic stress, and during exposure to the abscisic acid. Germination of plants has been analyzed and root growth was monitored on nutrient media with addition of 0.1-50 μM abscisic acid, 50-200 mM sodium chloride, 25-150 potassium chloride and 100-500 mM mannitol. It was shown that enhanced expression of BPM1 protein allows increased germination of seeds exposed to stress with regard to wild type plants. Enhanced expression of SerRS protein decreases germination of transgenic seeds. Root growth of transgenic lines did not significantly vary in comparison to wild type plants during stress exposure.

Item Type: Thesis (Diploma thesis)
Keywords: protein BPM1, Arabidopsis thaliana transgenic plants, salt stress, osmotic stress, ABA
Supervisor: Bauer, Nataša
Co-supervisor: Rokov Plavec, Jasmina
Date: 2013
Number of Pages: 45
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 26 Sep 2014 11:27
Last Modified: 26 Sep 2014 11:27
URI: http://digre.pmf.unizg.hr/id/eprint/2841

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