The regulation of heterologous ZmMAB1 protein expression in the transgenic Arabidopsis thaliana (L.) Heynh. lines

Šimac, Matija (2013) The regulation of heterologous ZmMAB1 protein expression in the transgenic Arabidopsis thaliana (L.) Heynh. lines. Diploma thesis, Faculty of Science > Department of Biology.

[img] PDF
Restricted to Registered users only
Language: Croatian

Download (2MB) | Request a copy

Abstract

Zea mays MATH-BTB protein (ZmMAB1) has been shown to have a role in regulation and proper asimetric cell divisions during the male and female gametophyte development. Its role has been demonstrated in proteasomal degradation as part of a ubiquitin E3 ligase complex. ZmMAB1 gene expression in Zea mays is precisely regulated, and the protein product is a short-lived protein. Therefore ZmMAB1 gene expression research in a homolougus system is difficult. Cullin is a structural component of many E3 ligases. It has been shown that Cul3a protein from Arabidopsis thaliana (L.) Heynh. forms complexes with MAB1 from Zea mays, so Arabidopsis thaliana has been chosen for expression regulation research of MAB1 in a heterologous system. For the cause of better understanding the role and regulation of ZmMAB1 protein, GFP florescent protein labeled ZmMAB1 protein has been inserted in the genome of A. thaliana with bacterium Agrobacterium tumefaciens. Goal of this thesis was to determine on which level of formation (posttranscriptionaly or posttranslationaly) ZmMAB1 protein is regulated. Results have shown that ZmMAB1 protein is undergoing ubiqutination and that way his levels in the cell are regulated.

Item Type: Thesis (Diploma thesis)
Keywords: MATH-BTB, ubiqutin, cullin, regulation of expression, proteasome, heterologous expression, asimetric cell division
Supervisor: Leljak Levanić, Dunja
Co-supervisor: Juranić, Martina
Date: 2013
Number of Pages: 51
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 26 Sep 2014 11:43
Last Modified: 16 Feb 2016 14:08
URI: http://digre.pmf.unizg.hr/id/eprint/2845

Actions (login required)

View Item View Item

Nema podataka za dohvacanje citata