Effect of integrin αv or α4 silencing on sensitivity of human breast cancer cells to vincristine and paclitaxel

Ferenčak, Krešimir (2013) Effect of integrin αv or α4 silencing on sensitivity of human breast cancer cells to vincristine and paclitaxel. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Integrins are heterodimeric transmembrane proteins that mediate the adhesion of cells to other cells and extracellular matrix. Besides regulation of proliferation, migration, metastasis and angiogenesis, integrins regulate cell sensitivity to antitumor drugs. In this study we determine the effect of integrin subunits αv, α4, α3, ß3 and ß5 silencing using integrin-specific siRNA in MDA-MB-435S breast cancer cells in sensitivity to antitumor drugs used in the treatment of breast cancer. αv, α3, ß3 and ß5 subunit silencing reduces sensitivity of cells to cisplatin, while not changing the sensitivity to doxorubicin and kamptotecin. However, αv, α4 and ß5 silencing increases cell sensitivity to vincristine and paclitaxel. It was also shown that the ability of cell migration reduces after αv silencing, and increases after α4 silencing. These results indicate that transfection of breast cancer cells expressing αv integrins with αv-specific siRNA could increase the effectiveness of vincristine and paclitaxel, and at the same time reduce the metastatic potential.

Item Type: Thesis (Diploma thesis)
Keywords: integrins, gene silencing, cytostatics, cell migration
Supervisor: Ambriović Ristov, Andreja
Date: 2013
Number of Pages: 64
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 29 Sep 2014 12:32
Last Modified: 29 Sep 2014 12:32
URI: http://digre.pmf.unizg.hr/id/eprint/2863

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