Microsatellite instability and hMSH2 gene analysis in sporadic colon cancer

Jukić, Patricia (2013) Microsatellite instability and hMSH2 gene analysis in sporadic colon cancer. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Approximately 15% of sporadic colorectal carcinomas (CRC) arise from mutator carcinogenic pathway which is characterized by microsatellite instability (MSI) caused by deficient mismatch repair system (MMR), secondary to defects in one of MMR genes. These tumors have distinct molecular and clinical-pathological features and different response to therapy. In this study we have examined the loss of heterozygosity (LOH) at the hMSH2 gene, a crucial gene in MMR system, by VNTR (D2S123 and D2S378) analysis and microsatellite instability (MSI) using the Bethesda panel of 5 microsatellite markers in 100 CRC patients. Results were compaired with clinico-pathological characteristics of patients and their tumors. LOH was detected in 9.4% and high MSI in 8.2% analysed tumors. There was no statictically significant correlation between the LOH/MSI and clinico-pathological characteristics, although LOH/MSI was more frequent in larger, poorly differentiated and advanced stage tumors.

Item Type: Thesis (Diploma thesis)
Keywords: hMSH2, sporadic colorectal cancer, LOH, MSI
Supervisor: Čačev, Tamara
Co-supervisor: Matulić, Maja
Date: 2013
Number of Pages: 43
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 30 Sep 2014 08:48
Last Modified: 30 Sep 2014 08:48
URI: http://digre.pmf.unizg.hr/id/eprint/2870

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