Cell cycle regulation by protein ubiquitination

Klasić, Marija (2009) Cell cycle regulation by protein ubiquitination. Bachelor's thesis, Faculty of Science > Department of Biology.

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Abstract

Cell cycle of eukaryotic cells has to be strictly regulated. During cell division, many morphological changes which are temporary and spatially precisely regulated occur to ensure equal distribution of the newly replicated copies of genome into the two daughter cells. Two mechanisms ensure cell cycle regulation: phosphorylation and targeted proteolysis. Targeted, ubiquitin-dependent proteolysis is regulated by three types of enzymes: E1, ubiquitin-activating enzyme, E2, ubiquitin-conjugating enzyme and E3, ubiquitin protein ligase. Most important E3 ligases which regulate cell cycle are APC/C, SCF and Cullin3-based E3 ligase complex. The APC/C is required for triggering the metaphase to anaphase transition and mitotic exit regulation. This complex degrades the separase inhibitor securin and mitotic cyclins. SCF complexes regulate early stages of mitosis and citokinesis by the control of Cdc2-cyclin B activity, a key regulator of cell cycle. It ensures G2 to M phase transition. Cullin3-based E3 ligases control chromosome position and citokinesis. Human Cullin3- KLHL9-KLHL-13 complex ensures chromosome attachment and progress of mitotis by degradation of Aurora B which dissociate from mitotic chromosome during chromosome alignment in prometaphase. Sister chromatids are held together by cohesin, which has to be eliminated for proper chromosome segregation in anaphase. Cyclin-dependend kinases (CDKs) inactivation is very important for mitotic exit. Many proteins, which are directly or indirectly connected, are involved in cell cycle regulation. In this network, it is very difficult to find out every single protein and its functions.

Item Type: Thesis (Bachelor's thesis)
Supervisor: Leljak Levanić, Dunja
Date: 2009
Number of Pages: 11
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 02 Oct 2014 11:17
Last Modified: 16 Feb 2016 14:08
URI: http://digre.pmf.unizg.hr/id/eprint/2903

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