Effects of salt stress on Opuntia streptacantha Lem. species

Paulišić, Sandi (2010) Effects of salt stress on Opuntia streptacantha Lem. species. Bachelor's thesis, Faculty of Science > Department of Biology.

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Salinity is one of the major factors limiting plant development and productivity, mainly of important crops. In arid and semiarid regions due to fresh water shortage, agriculture has increased usage of saline water. In salt stress and water deficit conditions, Pro is one of the most accumulated osmolytes in plants thus Pro content has been measured in such conditons. Expression level of Δ1-pyrroline-5-carboxylate synthetase (Osp5cs gene) and P5CS (γ-glutamyl kinase and glutamate-5-semialdehyde dehydrogenase) as a major enzyme of Pro biosynthetic pathway has been measured in O. streptacantha Lem. Salt stress treatment (6, 9 and 11 days) of O. streptacantha Lem. young plants resulted in a decrease of cladode thickness and root lenght, and in a significant and gradual accumulation of Pro depending of time and concentration. In general, salt stress has lead to P5CS (γ-glutamyl kinase) reduced activity, except at 75 and 150 mM NaCl at the sixth day. Osp5cs gene of O. streptacantha Lem. was induced by salt stress at ninth and eleventh day of treatment which has been confirmed by RT-PCR analysis. ABA also induced Osp5cs gene expression in young cladodes. Pro accumulation and Osp5cs gene transcription regulation revealed evident correlation between them under salt stress conditions without conferring with the changes in P5CS enzymatic activity. Pro might function as an osmolyte in salt stress conditons and might have a major role in osmotic regulation and photosyntetic activity protection of O. streptacantha Lem.

Item Type: Thesis (Bachelor's thesis)
Supervisor: Balen, Biljana
Date: 2010
Number of Pages: 12
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 06 Oct 2014 11:13
Last Modified: 06 Oct 2014 11:13
URI: http://digre.pmf.unizg.hr/id/eprint/2946

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