The use of recombinant DNA technology in producing pharmaceuticals

Tesla, Blanka (2010) The use of recombinant DNA technology in producing pharmaceuticals. Bachelor's thesis, Faculty of Science > Department of Biology.

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Abstract

Recombinant DNA technology encompasses various techniques by which we can transfer genes from one organism to another. This way we accomplished production of proteins in organisms for which these proteins are not natural. Such technology has many applications today. One of them is the use in producing pharmaceuticals. Proteins play an important role in the pharmaceutical industry. The first human protein produced in Escherichia coli was insulin, in 1982. Today, we use biotechnology in order to obtain recombinant proteins from bacteria, yeasts, animals, plants and cell cultures. Each manufacturing method has its advantages and disadvantages. The major drawback of bacteria is that they are unable to perform the posttranslation modifications such as glycosylation. Yeasts, as eukaryotes, have some advantages over bacteria in this process. Transgenic animals can secrete significant amounts of protein in blood, milk, egg white or urine. They can also produce proteins with complex structure that must undergo a process of posttranslational modifications. The use of transgenic animals faces many ethical issues and issues of environmental impact of such animals. Transgenic plants can produce large amounts of protein and they can also form complex protein structures. This system of production is cheap and avoids some ethical issues. The biggest disadvantage is interaction with the environment. Although we still know little about how transgenic organisms could affect the nature one day, we can say that the recombinant DNA technology in the production of pharmaceuticals is sort of a miracle of the 20th century.

Item Type: Thesis (Bachelor's thesis)
Supervisor: Pavlica, Mirjana
Date: 2010
Number of Pages: 15
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 06 Oct 2014 11:31
Last Modified: 06 Oct 2014 11:31
URI: http://digre.pmf.unizg.hr/id/eprint/2948

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