Next generation sequencing

Vučenović, Dunja (2013) Next generation sequencing. Bachelor's thesis, Faculty of Science > Department of Biology.

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Abstract

Information stored in DNA sequence contains valuable data about that organism, and by knowing ones genome, we will be able to understand processes happening in that being. Over the past few years, massively parallel DNA sequencing platforms have become widely available dramatically reducing the cost of DNA sequencing. The fast and low-cost sequencing approaches not only change the landscape of genome sequencing projects but also usher in new opportunities for sequencing in various applications. First developed massively-parallel sequencers were those that used clonally amplified fragments of DNA. By amplifying the fragments, sequencers would receive much stronger signal of incorporated nucleotide, but amplification leads to many errors causing less accurate final sequence. Later developed, third generation of sequencers uses single molecule templates and sequencing is usually done in real time. These methods are more accurate but also they can be used in quantitative applications. The newest and the most promising platforms are ones based on nanopore sequencing. Because of small dimensions of sequencing space, enormous amount of DNA could be sequenced in just one reaction, on one sequencer. These methods are still under development because of lot of problems designers of these machines encountered when doing in such small space. This thesis introduces into the high-throughput sequencing technologies, advantages and disadvantages of every platform and their biological application.

Item Type: Thesis (Bachelor's thesis)
Supervisor: Vlahoviček, Kristian
Date: 2013
Number of Pages: 16
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 15 Oct 2014 10:38
Last Modified: 15 Oct 2014 10:38
URI: http://digre.pmf.unizg.hr/id/eprint/3063

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