Structure and function of holoenzyme exemplified by bacterial RNA-polymerase

Bingula, Rea (2013) Structure and function of holoenzyme exemplified by bacterial RNA-polymerase. Bachelor's thesis, Faculty of Science > Department of Biology.

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RNA-polymerase together with the σ-subunit creates a holoenzyme capable of specific promoter sequence recognition. Thus it can commence important step of gene expression – transcription. Interwoven binding of promoter sequence, polymerase and σ-subunit leads to a number of allosteric modifications – exposure of binding sequences, salt bridge establishments, active site formation, specific structure de/stabilization , etc., all of which guarantee promoter-specificity of holoenzyme. Also, numerous intermolecular interactions with promoter bases maintain high conserved nucleotide sequence (A-11, T-7) in all living domains. One of the biggest discoveries certainly is the retaining of σ-subunit after the initiation. The complexity of regulation has increased even more since the same complex has to recognize the same sequences but with different affinity at different point in time, so the transition from one state to another can be achieved. Nowadays, techniques such as fluorescence probing, crystallography or mass spectrometry, lead to more precise organization or interaction determination inside the complex structures such as holoenzymes that will probably lead to many reconsiderations of what was thought to be dogmatic.

Item Type: Thesis (Bachelor's thesis)
Supervisor: Weygand-Đurašević, Ivana
Date: 2013
Number of Pages: 15
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 15 Oct 2014 10:55
Last Modified: 15 Oct 2014 10:55

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