Transcription – coupled repair

Markulin, Lucija (2012) Transcription – coupled repair. Bachelor's thesis, Faculty of Science > Department of Biology.

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Transcription-coupled DNA repair (TCR) is one of the subpathways of nucleotide excision repair (NER) that occurs when RNA polymerase is stalled at sites of DNA damage in the template strand. Template strand is repaired more quickly that similar lesions in coding strand or unexpressed parts of genome. Although the TCR was first observed in eukaryotes TCR phenomenon is best characterized in E. coli. The main role of TCR in bacteria has Mfd (ATP-dependent DNA translocase), also known as a transcription repair coupling factor (TRCF). Model for TCR postulate that Mfd recognizes and binds specifically to stalled and reverse translocated RNAP and using energy derived from ATP hydrolysis translocates forward RNAP to the transcription block. That results in RNAP release, transcript termination and recruitment of the NER machinery to the site of lesion. Recent studies show existence of alternative Mfd-independent TCR pathway mediated by elongation factor NusA. Alot is invested in understanding of more complex mechanism of TCR in eukaryotes due to the fact that mutations in genes associated with loss of TCR lead to severe disease in humans. The mechanism of TCR in humans is largely unknown despite having known some of the major participants in this process, such as CSA, CSB, TFIIH and XPG proteins. It is important to establish the mechanism of TCR not only because of the problems that stalled RNAP causes in the cell and diseases caused by dysfunctional TCR but also because the TRCF is involved in linking central cellular processes and knowing how TRCF uses its multiple domains to manipulate RNAP and the NER would help shed the light on the functional mechanism of TCR.

Item Type: Thesis (Bachelor's thesis)
Supervisor: Ivančić Baće, Ivana
Date: 2012
Number of Pages: 18
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 23 Oct 2014 09:18
Last Modified: 23 Oct 2014 09:18

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