Expression of intrinsically disordered proteins in normal and stressful conditions

Rončević, Tomislav (2014) Expression of intrinsically disordered proteins in normal and stressful conditions. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Intrinsically disordered proteins (IDPs) are involved in metabolic regulation, signalization, controlling pathways and in various types of biotic and abiotic stress. This study was conducted using N, HO and HNO sugar beet (Beta vulgaris L.) lines. We have successfully separated IDPs from globular proteins, and IDPs have been further analyzed using modified polyacrylamide gel electrophoresis. IDPs expression differed between cell lines indicating some IDPs are regulated by cell developmental stage. Isolated IDPs from the cell lines were tested whether they are N–glycosylated. Results are indicating that IDPs are not glycosylated, although this remains to be verified. HO line was chosen and treated with 300 mM mannitol and cadmium in three different concentrations (10 μM, 40 μM and 100 μM CdCl 2) to cause water deficit and heavy metal stress. Mannitol treatment caused phenotype changes, slower cell growth, increase of peroxidase activity, as well as a change in IDP expression compared with control. Highest cadmium treatment also led to responses on a phenotype level, increase of peroxidase activity and different protein expression compared with control. Lower cadmium concentrations were not sufficient to produce significant response to heavy metal phytotoxicity.

Item Type: Thesis (Diploma thesis)
Keywords: IDP, Beta vulgaris L., abiotic stress, cadmium, mannitol, water stress
Supervisor: Pavoković, Dubravko
Date: 2014
Number of Pages: 52
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 12 Nov 2014 13:31
Last Modified: 12 Nov 2014 13:31
URI: http://digre.pmf.unizg.hr/id/eprint/3244

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