Changes in expression of GFAP and PSA-NCAM epitopes after application of demyelinating substance cuprizone in mouse strain C57black/6

Zelen, Ada (2014) Changes in expression of GFAP and PSA-NCAM epitopes after application of demyelinating substance cuprizone in mouse strain C57black/6. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Treatment of a mice with cuprizone leads to massive apoptosis and demyelination of neurons. Demyelination with declined number of oligodendrocytes is also accompanied by the emergence of astroglia and microglia. During the process of demyelination surface of axolema is expressing PSA-NCAM epitopes which act as a negative regulator of myelination, preventing the binding of myelin creating cells (oligodendrocytes) on axons of neurons. PSA-NCAM also is a marker of stem cells and migration. Demyelination, with a decadence of stem cells follows the appearance of astrogliosis (increased number of reactive astrocytes). Astrocytes change their morphology and expression of proteins, including GFAP, commonly used immunohistochemical marker of reactive astrocytes. The aim of this study was to estimate the scope of neurogenesis and gliogeneze that occurs after applying a copper chelator cuprizone. The study was conducted on mice strains C57black / 6, age 3 months, who were fed with cuprizone for a period of nine weeks. Valuation of damage and regeneration capacity was made by immunohistochemical labeling with antibodies GFAP and PSA-NCAM in the region of the somatosensory cortex. From this study it can be concluded that the cuprizone leads to demyelination of neurons and that demyelination is accompanied by overexpression of PSA-NCAM epitopes and astrogliosis.

Item Type: Thesis (Diploma thesis)
Keywords: neurotoxin, demyelination, remyelination, neurogenesis, gliogenesis
Supervisor: Heffer, Marija
Co-supervisor: Đikić, Domagoj
Date: 2014
Number of Pages: 34
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 12 Nov 2014 13:37
Last Modified: 12 Nov 2014 13:37
URI: http://digre.pmf.unizg.hr/id/eprint/3245

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