The role of HLA-DPB1 alleles on outcome of hematopoietic stem cell transplantation from unrelated donor

Grba, Adriana (2014) The role of HLA-DPB1 alleles on outcome of hematopoietic stem cell transplantation from unrelated donor. Diploma thesis, Faculty of Science > Department of Biology.

[img]
Preview
PDF
Language: Croatian

Download (1MB) | Preview

Abstract

In the present study the correlation between HLA mismatches among recipient-unrelated donor in hematopoietic stem cell transplantation (HSCT) were analyzed. Tested group comprised of 57 pairs. The distribution of DPB1 alleles in the group of patients, unrelated donors and healthy controls was compared. The next goal was to analyze the number of mismatches at HLA-A,-B,-C, -DRB1, -DQB1 and -DPB1 loci between recipient-donor pairs and their correlation on the outcome of HSCT. HLA alleles were tested by Polymerase Chain Reaction and specific primers or probes (PCR-SSP and PCR-SSO methods). Among all three tested groups DPB1*04:01 allele was the most frequent (42.1%). No statistically significant difference in survival after HSCT was observed between patients with 0, 1, or 2 mismatches at DPB1 locus. In the group of 10/10 matched pairs significantly better survival was observed for patients with 2 DPB1 mismatches. There was no statistically significant difference in survival between patients with permissive and non-permissive DPB1 mismatches. For the final conclusion about the necessity of matching for HLA-DPB1 alleles it is required to enlarge the number of studies.

Item Type: Thesis (Diploma thesis)
Keywords: HLA- DPB1, bone marrow transplantation, GvHD
Supervisor: Grubić, Zorana
Date: 2014
Number of Pages: 36
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 13 Nov 2014 10:28
Last Modified: 13 Nov 2014 10:28
URI: http://digre.pmf.unizg.hr/id/eprint/3252

Actions (login required)

View Item View Item