Influence of silver nanoparticles on growth and appearance of oxidative stress in Allium cepa roots

Milošić, Anita (2014) Influence of silver nanoparticles on growth and appearance of oxidative stress in Allium cepa roots. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Nanoparticles became the subject of interest in science thanks to their unique electric, chemical and physical properties. Since they are commonly used in consumer products, there is a need to investigate their possible negative effect on living organisms and environment. The present study investigated the effect of silver nanoparticles (AgNP) on growth and appearance of oxidative stress in A. cepa roots. The toxicity of AgNPs and AgNO3 applied at the same concentrations (25, 50, 75, 100 and 500 μM) was also compared. The effect of three different organic coatings, AgNP-citrate, AgNP-PVP and AgNP-CTAB, was additionally analysed. The results showed that ionic silver inhibited root growth more strongly than AgNP. Malondialdehyde (MDA) content, as a parameter of oxidative stress, was increased after treatment with ionic silver and nanosilver AgNP-CTAB and decreased after treatment with nanosilver AgNP-citrate and AgNP-PVP. Protein carbonyl content was also decreased after treatment with ionic silver and AgNP-citrate, and no changes were observed after AgNP-PVP and AgNP-CTAB treatment. All of three investigated antioxidant enzymes (CAT, PPX, APX) showed changes in activity that could be a consequence of root exposure to ionic and nanosilver.

Item Type: Thesis (Diploma thesis)
Keywords: Allium cepa, antioxidative enzymes, lipid peroxidation, oxidative stress, protein carbonyls, silver nanoparticles
Supervisor: Balen, Biljana
Date: 2014
Number of Pages: 43
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 17 Nov 2014 11:32
Last Modified: 17 Nov 2014 11:32
URI: http://digre.pmf.unizg.hr/id/eprint/3286

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