Molecular characterization of Coleus blumei viroids from coleus (Solenostemon scutellarioides (L.) Codd)

Jezernik, Karlo (2014) Molecular characterization of Coleus blumei viroids from coleus (Solenostemon scutellarioides (L.) Codd). Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Viroids are pathogens simpler than viruses found only in plants where they can replicate without adverse effects or can cause virus-like symptoms. Viroid genome comprises one small (246-401 nt), non-coding, single stranded covalently closed RNA molecule. Viroids do not depend on helper viruses for spreading and have autonomous replication which makes them the smallest known RNA-replicons. Solenostemon scutellarioides (L.) Codd hosts several viroids from genus Coleviroid that have common Central Conserved Region (CCR) and are members of the familiy Pospiviroidae. The aim of this work was to investigate the occurrence of Coleviroids in selected S. scutellarioides plants and to identify detected viroid species. Total RNA was extracted from leaf samples of three S. scutellarioides cultivars and subjected to reverse transcription and polymerase chain reaction (RT-PCR) with the use of universal primers designed specifically for Coleviroid CCR. Amplicons of about 250 and 360 base pairs were cloned and analyzed with SSCP (Single-strand Conformation Polymorphism) method. The most representative products were sequenced. Obtained sequences were analyzed and revealed the presence of two Coleviroides, namely, CbVd-1 and CbVd-3, for the first time in Croatia.

Item Type: Thesis (Diploma thesis)
Keywords: Coleviroid, CbVd-1, CbVd-3, RT-PCR, SSCP
Supervisor: Škorić, Dijana
Date: 2014
Number of Pages: 59
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 24 Nov 2014 12:36
Last Modified: 24 Nov 2014 12:36
URI: http://digre.pmf.unizg.hr/id/eprint/3348

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