The role of neuropeptide Y (NPY) in the osteogenic differentiation

Matić, Igor (2013) The role of neuropeptide Y (NPY) in the osteogenic differentiation. Doctoral thesis, Faculty of Science > Department of Biology.

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Neuropeptide Y is expressed by osteoblast lineage cells and when administered in vitro can inhibit osteoblast lineage differentiation. We hypothesize that NPY, generated by mature osteoblast lineage cells, can control and modulate differentiation of osteoprogenitor cells. In this study we have evaluated the effects of bone-specific overexpression by generating Col2.3NPY-ires GFPtopaz transgenic mice. RT-qPCR and NPY EIA analysis showed increased expression of NPY in transgenic mice. Col2.3NPY mice had 25% lower trabecular volume and 7-10% lower trabecular thickness. Gene expression analysis in Col2.3NPY mice did not show any changes in expression levels of OC, BSP and DMP-1. Terminal osteoblast differentiation was impaired in Col2.3NPY mCOB cultures as indicated by a 6-fold decrease in mineralized area and decreased expression of all osteogenic markers. Our model has shown that locally expressed NPY plays an important role in regulation of bone mass. All these findings suggest that peripheral mechanism of the NPY signaling is important in bone remodeling.

Item Type: Thesis (Doctoral thesis)
Keywords: bone, bone remodeling, osteoblasts, neuropeptide Y
Supervisor: Marijanović, Inga and Kalajzić, Ivo
Date: 2013
Number of Pages: 96
Subjects: BIOMEDICINE AND HEALTH CARE > Basic Medical Sciences
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 06 Feb 2014 15:35
Last Modified: 22 Sep 2014 12:35

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