Multigene sequence analysis of 'Candidatus Phytoplasma mali' isolates

Norac-Kljajo, Jelena (2014) Multigene sequence analysis of 'Candidatus Phytoplasma mali' isolates. Diploma thesis, Faculty of Science > Department of Biology.

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Phytoplasma (genus 'Candidatus Phytoplasma') are bacterial pathogens that cause numerous diseases in plants. Because of their small size, lack of cell wall and the inability of cultured in vitro they are affiliated to the class Mollicutes. Phytoplasmas have extremely reduced genome (680 - 1600 kb) and lack many essential genes for different metabolic pathways. Life cycle of phytoplasma involves two hosts – plant and insects. Although in insects usually there usually do not cause negative effects, their effect on plants is often fatal. Attacking the plant cells phytoplasmas retain within phloem from which metabolites are taken directly for growth and multiplication. Species ' Ca. Phytoplasma mali' causes apple proliferation (AP) and it is one of the most economically important diseases of fruit trees in Croatia. The most common characteristic symptoms of AP are proliferation of axillar buds, growth of branches known as ''witches' broom'', yellowing or reddening of leaves with phloem necrosis together with small, broadly shaped and poorly flavored fruit. Amplification and RFLP analysis of 16S rRNA gene confirmed the presence of 'Ca. Phytoplasma mali' in samples collected from symptomatic apple trees and samples from insect-vector. From positive samples aceF and pnp genes were amplified by PCR using specific primers and sent for sequencing. Phylogenetic analyses of obtained nucleotide sequences revealed useful information about variability of ‘Ca. Phytoplasma mali’ isolates in Croatia and represent the first genotyping study of this pathogen in the country.

Item Type: Thesis (Diploma thesis)
Keywords: phytoplasma, plants, insects, gene, 16S rRNA, phylogenetic analysis
Supervisor: Šeruga Musić, Martina
Date: 2014
Number of Pages: 42
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 25 Nov 2014 10:13
Last Modified: 25 Nov 2014 10:13

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