The effect of non-ionizing radiation on DNA integrity of honeybee larvae Apis mellifera Linné, 1758

Pavelić, Ana (2014) The effect of non-ionizing radiation on DNA integrity of honeybee larvae Apis mellifera Linné, 1758. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

The use of mobile telephony has dramatically increased in the recent years, causing the non-ionizing radiation environmental pollution, which can have DNA damaging effects. Such damage can potentially have harmful effects on functioning of living organisms and ultimately lead to a decrease of exposed populations. Non-ionizing radiation is considered as one of the possible causes for the loss of bee colonies, which have an irreplaceable role in the ecosystem and economic activities. Therefore, the aim of this study is to estimate the genotoxic effect of non-ionizing radiation of 900 MHz on DNA integrity in honeybee larvae and to determine the protective effect of the honeycomb on the larvae exposed to electromagnetic fields. The results obtained by the Comet assay showed significant damage only in larvae exposed to modulated (1 kHz, 80%) electromagnetic with field strength of 23 V/m, while other exposures had no significant effect on DNA damage. The observed effects of radiation were of nonthermal nature, because we did not detect an increase in temperature due to exposure to radiation in GTEM chamber. Exposure in the comb had a protective effect of modulated radiation on the bee larvae. Results of this study indicate that non-ionizing radiation does not have a major impact on bee larvae and it is possible that it is not the major cause of honey bee colonies decline.

Item Type: Thesis (Diploma thesis)
Keywords: non-ionizing radiation, DNA damage, Apis mellifera, comet assay
Supervisor: Klobučar, Goran
Co-supervisor: Tlak Gajger, Ivana
Date: 2014
Number of Pages: 59
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 25 Nov 2014 11:31
Last Modified: 25 Nov 2014 11:31
URI: http://digre.pmf.unizg.hr/id/eprint/3364

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