Extended-spectrum beta-lactamase in the urinary isolates of Enterobacteriaceae

Sivec, Sara (2014) Extended-spectrum beta-lactamase in the urinary isolates of Enterobacteriaceae. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Many strains of Enterobacteriaceae are causing infections in humans, mostly of the urinary and reproductive system, and often cause gastrointestinal infections, while lately have become important cause of infections associated with the health care. Antibiotics are defined as pharmacological agents that can completely destroy the bacteria or stop their growth or reproduction without causing significant damage to the host organism. The group of antibiotics that act on the cell wall and membrane is beta-lactam antibiotics. These are: penicillins, cephalosporins and cephamicins, monobactams, carbapenems and beta-lactamase inhibitors. The emergence of bacterial resistance to antibiotics compromises the success of the antibiotic therapy and medical achievements. Beta-lactamases are bacterial enzymes that degrade by hydrolysis of amide bond in the beta-lactam ring of penicillins, cephalosporins and monobactams, producing acidic derivates without antibacterial activity. Beta-lactamases represents a major mechanism of resistance of bacterial pathogens in beta-lactam antibiotics. Extended spectrum beta-lactamases are plasmid encoded enzymes became by dott-mutation in blaTEM or blaSHV gene, or from chromosomal AmpC beta-lactamase Klyvera ascorbata by horizontal gene transfer and mutation. This study was obtained from 121 urinary isolates collected in the Cantonal Hospital in Zenica, Bosnia and Herzegovina. Sent samples were suspected of ESBL production and the prevalence does not coincide with the data from previous studies because these were related to the prevalence in the total population. Our results have much larger values. Samples were tested for sensitivity to various antibiotics, antibiograms were made, and conjugation tests were done with plasmids.

Item Type: Thesis (Diploma thesis)
Keywords: Enterobacteriaceae, beta-lactamases, extended spectrum beta-lactamase, bacterial resistance, antibiotic, beta-lactam antibiotics, hospital
Supervisor: Bedenić, Branka
Co-supervisor: Pavoković, Dubravko
Date: 2014
Number of Pages: 51
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 25 Nov 2014 12:27
Last Modified: 25 Nov 2014 12:27
URI: http://digre.pmf.unizg.hr/id/eprint/3369

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