DNA replication in secondary lymphoid tissue

Tadić, Vanja (2014) DNA replication in secondary lymphoid tissue. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Germinal centers (GC) of secondary lymphoid tissue follicles are extremely dynamic microenvironments, where B‐lymphocytes undergo clonal expansion, somatic hypermutation, positive and negative selection and class‐switch recombination. These are places where B‐lymphocytes grow to become long‐living memory B cells and plasma cells, which produce highly specific antibodies. Because of their function, GC cells have a high proliferating rate, which makes replication a key process in these areas of tonsils. In secondary lymphoid tissue, aside from germinal centers, it is easy to observe mantle zones which contain Blymphocytes entering germinal center and interfollicular zones which contain mostly T‐lymphocytes in search for dendritic cells with specific antigen. Goal of this study was to analyze the transcript levels of genes that code for replication proteins in three functionally different parts of tonsil. Ten tonsils gathered after the tonsillectomy from children with recurrent tonsillitis were used. Functionally different parts of tonsil were collected using laser‐microdissection technology; RNA was isolated and reversely transcribed into cDNA for QPCR analysis of DNMT1, PCNA, MCM2, MCM7, CDT1, EZH2, geminin and p300 transcripts. Results show that GC cells express significantly higher amount of EZH2 compared to mantle zone cells, and significantly higher amount of geminin compared to interfollicular zone cells. These differences in EZH2 and geminin expression point to processes in which these proteins take part in tumor cells originating from GC B‐lymphocytes, that could be used as targets in future therapies.

Item Type: Thesis (Diploma thesis)
Keywords: replication, germinal center, mantle zone, interfollicular zone, EZH2, geminin
Supervisor: Korać, Petra
Date: 2014
Number of Pages: 33
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 25 Nov 2014 12:43
Last Modified: 25 Nov 2014 12:43
URI: http://digre.pmf.unizg.hr/id/eprint/3371

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