Perls reaction- histochemistry of iron in liver

Kutle, Ivana (2010) Perls reaction- histochemistry of iron in liver. Bachelor's thesis, Faculty of Science > Department of Biology.

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Abstract

The role of iron in the body is very important and diverse. Iron is an essential element and we can devide it on hem and nonheme iron. The term nonheme iron includes heterogeneous types of complexes in which iron is loosely bound to low-molecular weight organic bases and proteins, unlike hem iron where this element is linked to the high molecular weight protoporphyrin ring. For histochemistry of nonheme iron dissolved in acid solution , in use are two histochemical methods: Perls and Turnbull method and other methods using iron chelators and dependent on the creation of insoluble particles of iron. The most commonly used in laboratories is a Perls method and it stains ferrous and ferric iron ions, while the Turnbull method is specific only to ferrous ions. However, perfusion Turnbull methods in vivo can clearly demonstrate the distribution of ferrous iron, particularly in the lysosomes. For electron microscopy Perls and Turnbull reactions are enhanced by DAB / silver / gold method for better visualization and iron sulfide staining methods as well as redox-active iron with hydrogen peroxide or DAB-om are also applicable for electon microscopy. Despite all the advantages of the described methods, quantitative measurements of iron in some samples are quite complicated and difficult feasible. Today, for the quantitative assessment of chelating Fe2 + showed the best method based on quenching fluorescence metal indicators (PG, SG, rodaina, kalceina, ...) and its dequenching of the known quantities of various divalent metal chelating treatment, described in section 5.2. All these methods have an important role in the diagnosis of various disorders and tissue damage.

Item Type: Thesis (Bachelor's thesis)
Supervisor: Lacković Venturin, Gordana
Date: 2010
Number of Pages: 17
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 04 Dec 2014 13:49
Last Modified: 16 Feb 2016 14:04
URI: http://digre.pmf.unizg.hr/id/eprint/3458

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