Biological effects of D- met-enkephalin isomer in a murine model of chemical liver injury induced by paracetamol

Martinić, Roko (2014) Biological effects of D- met-enkephalin isomer in a murine model of chemical liver injury induced by paracetamol. Doctoral thesis, Faculty of Science > Department of Biology.

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Peptides and proteins in mammals and higher organisms are composed of L-amino acids. The investigation of peptide-enantiomerism and the use of D-amino acids is important for the design of novel protein structures, with properties different from the native all L-forms. Lmet- enkephalin enantiomer exhibits strong protection in the mice model of acetaminophen induced hepatotoxicity. Howerver, there is currently no data available on the properties and biological effects of D-met-enkephalin enantiomer, and consequently we evaluated its hepatoprotective potential. D-enantiomer was shown to be protective in the dose range from 0.5 mg/kg to 20 mg/kg. Plasma AST, ALT and liver necrosis were significantly reduced in comparison to control animals treated with physiological saline (p>0.01). The best protection was observed in animals treated with a dose of 5 mg/kg. The effects of D-met-enkephalin were investigated from the standpoint of the receptor and peptide blockade. It was shown that they were probably mediated via δ and ζ opioid receptors, and blocked by naltrexone and antisense peptide. It is concluded that D-met-enkephalin induced hepatoprotection is comparable to L-met-enkephalin one.

Item Type: Thesis (Doctoral thesis)
Keywords: D-met-enkephalin, naltrexone, opioid receptors, antisense peptidies, enantiomers, paracetamol, hepatoprotection
Supervisor: Štambuk, Nikola
Date: 2014
Number of Pages: 94
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 09 Dec 2014 13:21
Last Modified: 09 Dec 2014 13:21

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