The role of cyclooxygenase isoforms in sporadic colorectal carcinogenesis

Catela Ivković, Tina (2014) The role of cyclooxygenase isoforms in sporadic colorectal carcinogenesis. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Cyclooxygenases are the key enzymes in prostanoid biosynthesis. Two isoforms exist: COX-1, associated with maintainance of physiological conditions in colon; and inducible COX-2 that is mainly related to various pathologies including colorectal cancer. The aim of our study was to examine the role of COX isoforms in development and progression of sporadic colorectal cancer, mechanisms of their regulation and whether regulatory polymorphisms in COX genes are related to cancer susceptibility, mRNA and protein expression. Our results show that COX-1 has protective, and COX-2 tumorigenic role in colorectal mucosa. Loss of COX-1 is very frequent in carcinogenesis, while COX-2 shows greater variation between samples. Higher COX-2 expression correlates to worse patient survival. We proved the role of regulatory polymorphisms in cancer susceptibility, and COX-2 expression. Protein expression correlates with mRNA expression, and there is no associtation with miR-200, which indicates transcriptional regulation as the key regulatory mechanism of COX expression.

Item Type: Thesis (Doctoral thesis)
Keywords: COX-1, COX-2, sporadic colorectal cancer, SNP, expression, miR-200
Supervisor: Kapitanović, Sanja
Date: 2014
Number of Pages: 146
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 12 Dec 2014 09:24
Last Modified: 12 Dec 2014 09:24
URI: http://digre.pmf.unizg.hr/id/eprint/3498

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