Monitoring the processes of malignant modifications of prostate and liver cells

Šrajer Gajdošik, Martina (2014) Monitoring the processes of malignant modifications of prostate and liver cells. Doctoral thesis, Faculty of Science > Department of Chemistry.

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Abstract

The critical molecular and cellular mechanisms involved in the development and progression of prostate and liver cancer remain elusive. In this work we demonstrated that normal rat prostate epithelial cells (PEC) undergo spontaneous transformation at high passage (p>85) evidenced by the acquisition of anchorage independent growth and tumorigenicity when injected into immunodeficient mice. Minor subpopulation of these cells (SAI) had the ability to migrate through soft agar and showed marked differences in cell morphology, proliferation, motility and expression of signaling proteins as well as higher tumorigenic potential. In addition, we examined changes between released extracellular vesicles (EV) from stem-like liver epithelial cell line WB-F344 (WB), their chemically transformed GP7TB and GP7TB.SAI, a soft agar invasive subpopulation. Apart from changes in number, size and proteomic content of EV, we discovered that GP7TB.SAI derived EVs significantly stimulated NK-mediated cytotoxicity opposite to those purified from the GP7TB line, despite their higher expression of Necl-5.

Item Type: Thesis (Doctoral thesis)
Keywords: extracellular vesicles, prostate epithelial cells, hepatocellular carcinoma, malignant transformation, proteomics, tumorigenicity
Supervisor: Josić, Đuro
Date: 2014
Number of Pages: 146
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Faculty of Science > Department of Chemistry
Depositing User: mag. bibl. Ana Šafran
Date Deposited: 12 Jan 2015 12:31
Last Modified: 15 Jun 2015 12:59
URI: http://digre.pmf.unizg.hr/id/eprint/3588

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