Genetic diversity of major histocompatibility complex class II in horses naturally infected with pathogenic bacteria of the genus Leptospira

Mojčec Perko, Vesna (2014) Genetic diversity of major histocompatibility complex class II in horses naturally infected with pathogenic bacteria of the genus Leptospira. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Leptospirosis is a zoonotic disease of worldwide distribution caused by pathogenic bacteria of the genus Leptospira. High seroprevalence of leptospirosis was determined in horse population of Croatia. The major histocompatibility complex class II (MHC II) has a crucial role for patogen presentation to cells of the immune system and initiation of the immune response. The main carrier of genetic variability of MHC II region is exon 2. The genetic diversity and evolutionary history of the exon 2 DRA and DRB loci were analysed in 200 horses, 100 leptospira infected and 100 uninfected. Five DRA and 14 DRB alleles were detected, while six DRB alleles were new. Each allele results in a single amino acid sequence. 41 haplotypes were found. Highly diverged allele sequences and occurrence of trans-species polymorphism provide evidence for action of balancing selection on both loci. The statistical analyzes showed some indications of the rules of grouping individuals according to haplotype and leptospira serovar in which an individual shows serologic response, but due to the small number of individuals in a particular category is not possible to apply certain rules to the entire population of horses.

Item Type: Thesis (Doctoral thesis)
Keywords: Equus caballus, leptospirosis, MHC, DRA, DRB
Supervisor: Turk, Nenad and Galov, Ana
Date: 2014
Number of Pages: 182
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 19 Jan 2015 11:44
Last Modified: 19 Jan 2015 11:44
URI: http://digre.pmf.unizg.hr/id/eprint/3592

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