Activity of paraoxonase 1 in premalignant lesion of the cervix

Butorac, Dražen (2015) Activity of paraoxonase 1 in premalignant lesion of the cervix. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Paraoxonase 1 (PON1) is the enzyme that removes carcinogenic radicals of lipid peroxidation. The aim of this study was to determine the paraoxonase and arylesterase activity of PON1, phenotype distribution and polymorphisms of pon1 gene, and parameters of lipid status as well as oxidation stress in patients with premalignant lesion of the cervix. Basal and NaCl-stimulated paraoxonase activity of PON1 as well as those activities standardized on the concentration of HDL and apoAI did not differ significantly between control group and group of patients with cervical intraepithelial neoplasia (CIN). On the other hand, arylesterase activity of PON1 as well as activity standardized on the concentration of HDL and apoAI was significantly reduced in CIN group, and in the subgroups with low grade and high grade changes. Distribution of PON1 phenotypes was similar in the study groups. In addition distribution of genotypes and alleles of Q192R, L55M and -108C>T polymorphisms of pon1 gene did not differ between the study groups. Concentration of thiol groups was similar, of malondialdehide was lower and of glutathione was significantly higher in the CIN groups. Observed results indicated an oxidation-antioxidation disbalance in women with CIN. Decreased arilesterase PON1 activity might reflect reduced enzyme concentration which could be the consequence of down-regulated gene expression due to redox homeostasis disturbances.

Item Type: Thesis (Doctoral thesis)
Keywords: paraoxonase 1, polymorphism, cervical intraepithelial neoplasia, oxidative stress
Supervisor: Grdić Rajković, Marija and Kuna, Krunoslav
Date: 2015
Number of Pages: 121
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 04 Mar 2015 09:32
Last Modified: 04 Mar 2015 09:32
URI: http://digre.pmf.unizg.hr/id/eprint/3714

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