Molecular epidemiology of grapevine phytoplasma pathosystems: multilocus sequence typing approach

Plavec, Jelena (2015) Molecular epidemiology of grapevine phytoplasma pathosystems: multilocus sequence typing approach. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Phytoplasmas ('Candidatus Phytoplasma') are non-cultivable wall-less bacteria found in plant phloem and insect vector cells. Economically important Grapevine yellows (GY) diseases in Croatia are caused by phytoplasmas belonging to the ribosomal groups 16SrV-C and -D (flavescence dorée; FD) and 16SrXII-A (stolbur; bois noir; BN, ‘Candidatus Phytoplasma solani’) which cause similar symptoms but differ considerably in epidemiology. The aim of this thesis was to analyse BN and FD phytoplasma genetic variability through multilocus sequence typing (MLST) of molecular markers differing in conservation and evolution from infected grapevine (Vitis vinifera L.), wild plants and insect vectors collected in Croatia. MLST analysis of BN phytoplasma isolates revealed the presence of 17 different genotypes encompasing three tuf, five secY, seven vmp1 and ten stamp genotypes. The analysis of FD phytoplasma isolates demonstrated the existence of two map, four uvrB-degV and five secY genotypes. The vmpA gene analysis discriminated three clusters. This is the first multigene genotyping performed on FD phytoplasma isolates and first extensive research on BN phytoplasma isolates in Croatia. Results obtained through this comprehensive four-year research revealed considerable genetic diversity of epidemiological relevance and the geographical distribution of differentiated grapevine phytoplasmas strains. Furthermore, possible paths of pathogen introduction in Croatia are indicated.

Item Type: Thesis (Doctoral thesis)
Keywords: grapevine yellows, FD phytoplasma, BN phytoplasma, MLST, molecular markers, genotype, molecular epidemiology
Supervisor: Šeruga Musić, Martina and Foissac, Xavier
Date: 2015
Number of Pages: 169
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 04 Mar 2015 09:57
Last Modified: 04 Mar 2015 09:57
URI: http://digre.pmf.unizg.hr/id/eprint/3718

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