Expression of sodium-glucose cotransporter Sglt1 (Slc5a1) in murine organs

Vrhovac, Ivana (2015) Expression of sodium-glucose cotransporter Sglt1 (Slc5a1) in murine organs. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

In mice, sodium-glucose cotransporter (mSglt1/Slc5a1) absorbs and reabsorbs the glucose in the brush-border membrane of small intestinal enterocytes and renal proximal tubules, respectively. Due to unknown localization of mSglt1 in other organs/tissues, mSglt1 expression was determined comparing wild type (Sglt1+/+) and Sglt1 knockout mice (Sglt1-/-) at the mRNA and protein level. By qRT-PCR, mSglt1 mRNA expression was observed the highest in the small intestine; high in seminal vesicles, kidneys, salivary glands (parotid, submandibular, sublingual); medium in prostate, tongue, eyes and uterus; and small in pancreas, lung and liver. Immunofluorescence revealed detailed mSglt1 cell localization in the kidneys, small intestine, liver, pancreas, salivary glands, tongue, prostate, ulbourethral gland, seminal vesicles and uterus. In the kidneys, sex-dependent expression of mSglt1 was observed at mRNA and protein level. mSglt1 distribution in various mouse organs/tissues enables further studies of its role in patho/physiological, pharmacological and toxicological investigation in the most used experimental animals.

Item Type: Thesis (Doctoral thesis)
Keywords: imunnofluorescence, mouse organs, sex differences, Sglt1, qRT-PCR, western blot.
Supervisor: Breljak, Davorka
Date: 2015
Number of Pages: 128
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 10 Apr 2015 10:31
Last Modified: 10 Apr 2015 10:31
URI: http://digre.pmf.unizg.hr/id/eprint/3803

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