Moltkia petraea and Micromeria croatica plant extracts influence on protection of telomeres, senescence and proliferation of MJ90 cells

Cedilak, Matea (2015) Moltkia petraea and Micromeria croatica plant extracts influence on protection of telomeres, senescence and proliferation of MJ90 cells. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

The main role of telomeres is the protection of the chromosome structure. They also protect the chromosome ends from fusions, and since they shorten with every cell division they serve as counters of population doublings. Once the telomeres reach their critical length, the cell enters permanent and irreversible cell cycle arrest called senescence which makes them the most important regulators of the cellular ageing. By protecting the telomeres we could prevent their abrrupt shortening and also postpone the cell senescence. Effect of the plant extracts Micromeria croatica and Moltkia petraea on the protection of telomeres and therefore on the cell growth and ageing, was the main goal of this research. Cells were treated with the extracts for a longer period of time in the cell culture. SA-β-gal staining was used to evaluate the cell senescence, and BrdU labelling to examine cell proliferation. The telomere length was measured using Southern blot analysis. Cells treated with plant extracts exibited lower number of senesced cells and slightly longer telomeres compared to the non-treated control cells. This supports the conclusion that the extracts inhibit cellular aging, probably through the protection of the cell DNA and protein structures.

Item Type: Thesis (Diploma thesis)
Keywords: telomere shortening, senescence, M. croatica and M. petraea extracts
Supervisor: Rubelj, Ivica
Co-supervisor: Matulić, Maja
Date: 2015
Number of Pages: 54
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 16 Apr 2015 09:35
Last Modified: 16 Apr 2015 09:35
URI: http://digre.pmf.unizg.hr/id/eprint/3816

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