Influence of epigallocatechin-3-gallate on okadaic acid-induced cell death

Kopčok, Vedrana (2015) Influence of epigallocatechin-3-gallate on okadaic acid-induced cell death. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Okadaic acid is a selective inhibitor of serine/threonine phosphatases (PP1 and PP2A) that catalyze dephpsphorylation of tau protein and induce cdk5 and p25 (first write in full) expression. Cdk5 inhibits phosphatases and induces hyperphosphorylation of tau protein. Okadaic acid induces pathological changes of tau protein in vivo and in vitro in a way similar to that seen in Alzheimer’s disease (AD). Epigallocatechin-3-gallate (EGCG) is flavonoid found in green tea and is considered as one of the potentially neuroprotective agents for AD. EGCG is antioxidant and anti-inflamatory agent, and recent studies showed that it has several neuroprotective mechanisms of action. The aim of this work was to find out if EGCG can stop cell death induced by okadaic acid in neuroblastoma cell line SH-SY5Y and neurons derived therefrom. We have shown that EGCG pre-treatment and co-treatment with okadaic acid can decrease okadaic acid toxicity neuroblastoma SH-SY5Y cells and neurons differentiated therefrom.

Item Type: Thesis (Diploma thesis)
Keywords: Alzheimer’s disease, tau phosphorylation, EGCG
Supervisor: Šimić, Goran
Co-supervisor: Marijanović, Inga
Date: 2015
Number of Pages: 39
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 17 Apr 2015 08:44
Last Modified: 17 Apr 2015 08:44
URI: http://digre.pmf.unizg.hr/id/eprint/3825

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